. 2021 Oct 25;119(1):162–175. doi: 10.1002/bit.27961

Table 3.

Titer, product quality, and conjugation of aCD74 ADC produced in batch and continuous extract

		Batch extract	Continuous extract
CF titer of ProA capture by A₂₈₀		0.45 ± 0.073 g/L^a, ^b	0.56 ± 0.033 g/L^a, ^b
SEC purity of ProA pool	% Monomer	74%	88%
	% High MW	19%	5%
	% Low MW	7%	8%
SEC purity of polished mAb	% Monomer	98%	97%
	% High MW	0%	0%
	% Low MW	2%	2%
SEC purity of conjugated ADC	% Monomer	96%	96%
	% High MW	2%	2%
	% Low MW	3%	3%
ADC DAR by MALDI‐TOF MS		1.96	1.95

Abbreviations: ADC, antibody–drug conjugate; CF, cell free; DAR, Drug Antibody Ratio; MALDI‐TOF MS, matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry.

^{^a}

Titers are listed ± 1 standard deviation.

^{^b}

Batch and continuous titers are not significantly different with p > 0.08.