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P2X7R and NLRP3 colocalization in IVD cells. Average Manders colocalization coefficients (±SEM) were evaluated in control cells (CTR) or after treatment with 10 µg/ml LPS + 500 µM BzATP. *p < 0.0001. Representative images were also reported. P2X7R (Alexafluor 488, green); NLRP3 (Alexafluor 546, red). Scale bars = 10 μm. IVD, intervertebral disc; LPS, lipopolysaccharide
