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Cytotoxic activity and membrane effect of vCPP2319 in primary cell line from brain endothelium. The cytotoxic activity (A) of vCPP2319 toward HBMEC was evaluated (n = 4). Zeta potential measurements were performed in HBMEC treated with vCPP2319 at 5.2 μM (B) (n = 2). Error bars refer to the standard deviation obtained from different experiments, performed in different days with independently grown cell cultures. Significance was assessed by the unpaired t‐test. **P‐value ≤ 0.01.
