TABLE 5.

Recommendations for future preclinical studies investigating renal metabolism following ischemia reperfusion (IR)

Recommendation	Rationale
Functional IR injury definition	Consensus on renal dysfunction
Renal replacement therapy	Exclusion of prerenal causes of renal IR injury
Optimal ischemia time (titration of IR injury)	Chance for both transient dysfunction, that is, DGF, and ischemic controls, that is, primary function
Representative study population	Higher incidence of clinical renal IR injury in older patients
Renal vein catheterization (excludes use of mice)	Arteriovenous kidney‐specific sampling; metabolite diffusion
Successive sampling	Monitoring dysfunction and functional recovery
Extended follow‐up time	Monitoring dysfunction and functional recovery
Predefined set of metabolites	Insight in metabolic competence and straightforward comparisons