Figure 5.

Lysosomal proteolysis, but not proteasomal proteolysis, is necessary to regulate the abundance of nucleocytosolic acetyl-coA and fatty acid production enzymes. (A) Western blots for indicated proteins in lysates from NIH3T3 cells treated for 48 h with leupeptin and ammonium chloride to block lysosomal, but not proteasomal proteolysis. (B) Quantifications of western blots shown in (A). (C) Western blots for indicated proteins in lysates from NIH3T3 cells treated for 24 h MG132 to block proteasomal, but not lysosomal proteolysis (48 h treatments with MG132 were cytotoxic). (D) Quantifications of western blots shown in (C). Each experiment was repeated six times, with individual values shown on the graphs. Black bars indicate means and SEM. p values shown on graphs are derived by unpaired t-tests with Welch’s correction for unequal SDs. All proteins are adjusted for loading, using H3 as a control.