Fig. 4.

Effects of curculigoside and EAF on GLUT4 protein expression in differentiated 3T3-L1 adipocytes. (a) Representative immunoblot images. Relative protein expression of (b) GLUT4 in the total cell lysate and (c) GLUT4 in the plasma membrane of treated cells. α-Tubulin and IR-β were respectively being used as the loading control for total cell lysate and plasma membrane preparations. Values are expressed as mean ± standard deviation (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 compared to the control; #p < 0.05 and ##p < 0.01 compared to the insulin control.