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Composition of PHAMCL accumulated by Tn5 mutants of P. putida. Cultivations were performed under PHA-accumulating conditions on MM containing 1.5% (wt/vol) sodium gluconate and 0.05% (wt/vol) ammonium chloride. Cells were grown for 48 h at 30°C. PHA content and composition of comonomers were analyzed by GC. 3HHx, 3-hydroxy-hexanoate; 3HO, 3-hydroxyoctanoate. An asterisk indicates that cells harbor plasmid pBHR81. Plasmid pBHR81 enables functional expression of the phaGPp gene.
