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One‐pot enzymatic phosphorolysis‐transglycosylation reaction for the synthesis of nucleoside analogues. The first reaction step may be catalyzed by a pyrimidine nucleoside phosphorylase (PyNP) or a purine nucleoside phosphorylase (PNP) depending on the nucleobase (B1) of the sugar donor. B2 corresponds to a purine nucleobase.
