Table 1.

Phosphorolysis activity of HePNP with purine and pyrimidine nucleosides.^[a]

Substrate	Specific activity [U mg−1]	Phosphorolysis after 4 h [%]
inosine	20±0.4	57±2
2′‐deoxyinosine	44.9±1.5	54±3
adenosine	11±0.6	40±1
2′‐deoxyadenosine	20.4±2.1	45±1
guanosine	23.9±0.2	23±1
thymidine	1.2±0.1	4±0
5‐methyluridine	n.d.[b]	n.d.
uridine	2.3±0.3	6±1
2′‐deoxyuridine	n.d.	n.d.
2′‐deoxycytidine	n.d.	n.d.

[a] Reactions were carried out at 5 mm scale in 100 mm phosphate buffer pH 7.5 at 37 °C with 2 units of HePNP. [b] n.d.=not detected.