Table 2.

Immobilization parameters obtained for the different supports and chemistries.^[a]

Material support	Reactive group	Matrix	Immobilization yield[b] [%]	Recovered activity[c] [%]	Immobilized activity[d] [U g−1 support]
Ep−AG	epoxy/Co2+	agarose	93	38	7.0
EP403/S	epoxy/Co2+	methacrylate	97	26	5.0
Ep−Si2200	epoxy/Co2+	silica	100	18	4.0
Ep−Si250	epoxy/Co2+	silica	99	20	3.6
Gx−AG	aldehyde	agarose	99	29	5.7
SH−AG	thiol	agarose	94	37	6.9

[a] The protein offered was 1 mg g⁻¹. Ep−AG: epoxy−agarose, EP403/S: epoxy−methacrylate; Ep−Si2200 and EpSi250: epoxy−silica; Gx−AG: epoxy−agarose; and SH−AG: thiol−agarose. [b] Immobilization yield was calculated as: (protein in solution−protein in the supernatant after immobilization)/protein in solution ×100. [c] Recovered activity: specific activity of the free enzyme/specific activity of the immobilized enzyme ×100. [d] Immobilized activity on the support: activity of the immobilized enzyme/g of support.