Table 3.

Biosynthesis of nucleoside analogues by the immobilized HePNP.^[a]

Entry	Nucleobase	Transglycosylation [%]
		batch (24 h)	flow (2 min)	flow (10 min)	flow (30 min)
1		80±2.1	56±1.2	91±2.1	n.t.
2		29±5.4	7±2.3	15±3.2	14±3.1
3		30±2.6	6.9±1.6	12±3.4	21±0.6
4		65±0.1	2.4±0.2	17±2.8	54±2.8
5		56±2.9	1.9±0.1	14±1.8	19±1.1

[a] The reactions were performed with 25 mm inosine and 5 mm nucleobase in 20 mm phosphate buffer at pH 7.5 and 37 °C. Batch reactions were carried out in 2 mL with 2 U of enzyme. For the flow reactions, the volume of the PBR (4 mg g⁻¹ immobilized enzyme) was 1.7 mL. Retention times are depicted. n.t.=not tested.