Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 24;23(2):e202100472. doi: 10.1002/cbic.202100472

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Modular surface display of a BM3 passenger employing different membrane anchors. (A) The membrane anchor is provided with a SC and the BM3 passenger with a matching ST binding unit. Both parts are expressed separately from individual plasmids, post‐translationally assembled through the specific, covalent SC/ST interaction and the complex is finally installed in the E. coli cell envelope via the membrane anchor. (B) Due to the modularity of the system, the different membrane anchors Lpp‐OmpA (I), PgsA (II), INP_NC (III) and AIDA‐I (IV) can be compared regarding their performance for surface display of a BM3‐ST passenger. (C) Schematic representation of genetic constructs for the different membrane anchors fused to SC domains via flexible GGGGS linkers. CRD: central repeat domain; ctxB SP: signal peptide of cholera toxin B subunit.