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. 2022 Jul 18;2(7):e465. doi: 10.1002/cpz1.465

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© 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Results of a microneutralization assay against B/Brisbane/60/2008 using samples with variable titers. (A) Results for a sample plate. Sample number and microneutralization titer (MNT) are shown in the two left columns. As shown on the x axis, serum samples were first diluted 1:10 and then serially diluted 1:2. A lack of agglutination is indicated by a cell pellet at the bottom of the plate, whereas agglutination is indicated by a homogenous cloudy reddish cell suspension. (B) Controls used for experiment in (A). (C) Representation of plotting and neutralization titer visualization showing eight positive samples from panel A plus four negative samples. The limit of detection (LoD) of the assay is indicated by a dotted line. The negative samples are displayed below the LoD and positive samples are shown equal to or above the LoD. Reciprocal titers (1:x serum dilution) are displayed as absolute values.