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. 2022 Jul 6;13:931630. doi: 10.3389/fimmu.2022.931630

Figure 5.

Figure 5

Loss of NKG7 promotes hypersecretion of cytokines following immune synapse formation. (A–D) RNA-sequencing of 72 hour activated Nkg7-/- and Nkg7+/+ CD8+ T cells following co-culture with anti-CD3/28-coated P815 cells for 4 hours. (A) Volcano plot of genes up or down regulated in Nkg7-/- cells compared to Nkg7+/+ cells, with blue indicating genes with a P-value < 0.05, FC – fold change (B) Heatmap of top 30 differentially expressed genes. (C) Functional Gene Ontology (GO) enrichment analysis of the top 100 differentially upregulated genes. (D) STRING network analysis of top 50 differentially upregulated genes, showing connected nodes only. (E–G) Cytokines secreted by 72 hour activated Nkg7-/- or Nkg7+/+ CD8+ T cells co-cultured with anti-CD3/28-coated P815 tumor cells for 4 hours, measured by antibody-pair-based array (E, F) or cytokine bead array (G) using supernatants from co-cultures. (E) Columns represent replicates pooled from 2 independent experiments. (F) Red shows cytokines with average fold change >1.5; blue shows cytokines with average fold change >1, <1.5; error bars show +/- SEM. (G) Unpaired t test, n = 6; ns – not significant, ** P < 0.001.