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. 2021 Nov 17;119(2):470–481. doi: 10.1002/bit.27984

Table 4.

Kinetic parameters of Thc_Cut1 and Thc_Cut2 on ETE, pNP‐C2, pNP‐C4, pNP‐C8, pNP‐C12, and pNP‐C16^a

	K_M (mM)	k_cat = ^convV_max/E₀ (s⁻¹)	k_cat/K_M (mM⁻¹·s⁻¹)
Thc_Cut1
ETE	0.99 ± 0.20	2.6 ± 0.26	2.7 ± 0.61
pNP‐C2	2.31 ± 0.57	0.34 ± 0.02	0.15 ± 0.03
pNP‐C4	0.25 ± 0.01	0.17 ± 0.00	0.70 ± 0.03
pNP‐C8	0.31 ± 0.02	0.21 ± 0.00	0.66 ± 0.04
pNP‐C12	0.07 ± 0.02	0.08 ± 0.00	1.19 ± 0.30
pNP‐C16	0.03 ± 0.00	0.10 ± 0.02	3.18 ± 0.45
Thc_Cut2
ETE	ND^b	ND	0.059 ± 0.0012^c
pNP‐C2	4.56 ± 0.30	0.44 ± 0.03	0.10 ± 0.01
pNP‐C4	0.43 ± 0.11	0.16 ± 0.01	0.38 ± 0.14
pNP‐C8	0.40 ± 0.03	0.26 ± 0.01	0.63 ± 0.01
pNP‐C12	0.05 ± 0.01	0.10 ± 0.02	1.90 ± 0.50
pNP‐C16	0.07 ± 0.01	0.09 ± 0.01	1.40 ± 0.22

^{^a}

Data represent average values and the spread of duplicate experiment.

^{^b}

ND, Not detected; could not be saturated up to 2 mM ETE.

^{^c}

Calculated from the slope of linear regression.