Figure 1.

a) Polyacrylamide gel analysis of the commercially obtained RNA building blocks RNA1–RNA4 and their 3′‐extended products obtained in solution and after HPLC‐purification. b) Polyacrylamide gel analysis of ligations on the solid support. Ligation 1: RNA1‐C^NPE +p‐RNA2‐U^NPE , ligation 2: RNA1‐C^NPE‐RNA2‐U^NPE +p‐RNA3‐C^NPE‐RNA4‐G^NPE , ligation 3: RNA1‐C^NPE‐RNA2‐U^NPE‐RNA3‐C^NPE‐RNA4‐G^NPE +p‐RNA5. c–e) Total yields in the three synthetic strategies explored in this study. The values given are for the synthesis with incorporated NPE‐modified nucleosides. The yields for the synthesis with mAzo C‐nucleosides were similar (see text).