TABLE 1.

Number of animals included in different experimental groups and various parameters.

Groups	ECG, and hemo-dynamics tests	Evans blue-TTC staining	ELISA for plasma		Proteomic analysis	Western blotting	TEM, IF for ROS	Total
			HE, TUNEL	ELISA for left ventricle
Sham	6	6	3	6	3	4	3	31
I/R	7	6	3	6	3	4	3	32
HS1 + I/R	7	6	3	6	0	4	3	29
HS2 + I/R	0	6	3	6	0	4	3	22
HS4 + I/R	6	6	3	6	3	4	3	31
Total	26	30	15	30	9	20	15	145

The parameters in a column used the same animals from each group for assessment. Sham, Sham group; I/R, I/R 120 min group; HS1 + I/R, pre-treatment with HS at 1 g/kg plus I/R 120 min group; HS2 + I/R, pre-treatment with HS at 2 g/kg plus I/R 120 min group; HS4 + I/R, pre-treatment with HS at 4 g/kg plus I/R 120 min group; ECG, electrocardiograph; TTC, 2, 3, 5-triphenyltetrazolium chloride; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; ELISA, enzyme-linked immunosorbent assay; TEM, transmission electron microscope; IF, immunofluorescence; ROS, reactive oxygen species.