Table 1.
Primer sequences and conditions of PCR.
| cDNA | GenBank | Forward and reverse primers | Ta (°C) | Product (pb) |
|---|---|---|---|---|
| RNAPol2 | NM_000937 | F: 5’-CCAAGCAGGACGTAATAGAGG-3’ R: 5’-GCGGTCTAGCAACTCGTTCT-3’ |
55 | 193 |
| PPIA | NM_021130 | F: 5’-ACCGTGTTCTTCGACATTGC-3’ R: 5’-CAGGACCCGTATGCTTTAGGA-3’ |
55 | 274 |
| PPARγ2 | NM_015869 | F: 5’-CAAACCCCTATTCCATGCTGTT-3’ R: 5’-AATGGCATCTCTGTGTCAACC-3’ |
53 | 135 |
| C/EBPα | NM_004364 | F: 5’-ACTGGGACCCTCAGCCTTG-3’ R: 5’-TGGACTGATCGTGCTTCGTG-3’ |
55 | 75 |
| IL6 | NM_000600 | F: 5’-CAATGAGGAGACTTGCCTGG-3’ R: 5’-GCACAGCTCTGGCTTGTTCC-3’ |
53 | 113 |
| PLIN 1 | NM_002666 | F: 5’-GAGACACTGCGGAATTTGC-3’ R: 5’-ATCGAGAGAGGGTGTTGGTC-3’ |
58,2 | 222 |
| Ywhaz | NM_145690 | F: 5’-GGTCATCTTGGAGGGTCGTC-3’ R: 5’-GTCATCACCAGCGGCAAC-3’ |
55 | 245 |
| Runx2 | AF001450 | F: 5’-GCTGTTATGAAAAACCAAGT-3’ R: 5’-GGGAGGATTTGTGAAGAC-3’ |
60 | 108 |
| OC | NM_199173 | F: 5’-ATGAGAGCCCTCACACTCCTC-3’ R: 5’-GCCGTAGAAGCGCCGATAGGC-3’ |
55 | 293 |
| Dlx5 | BC006226 | F: 5’-CCAGAGAAAGAAGTGACCGA-3’ R: 5’-CCTGTGTTTGTGTCAATCCC-3’ |
55 | 190 |
The primer sequences, annealing temperatures (Ta), lengths of the corresponding PCR products and GenBank accession numbers are shown. F, Forward; R, Reverse. IL6, Interleukin 6; PLIN 1, Perilipin 1; C/EBPα, CCAAT/enhancer-binding protein alpha; PPARγ2, Peroxisome proliferator-activated receptor gamma 2; PPIA, peptidylpropyl isomerase; Runx2, Runt-related transcription factor 2; Ywhaz, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; OC, Osteocalcin; Dlx5, Distal-less homeobox 5.