Figure 3.

The pan-PI3K inhibitor LY294002 accelerates neutrophil death. (A, B) The effect of LY294002 on apoptosis of control and LVS-infected neutrophils was assessed at 4, 10, 18, and 24 hours by Annexin V-FITC/PI staining and flow cytometry (A) and at 18 hours by analysis of nuclear morphology (B). Data are the mean ± SEM of three independent experiments. ns, not significant. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, as indicated. (C) Immunoblots of cell lysates show that LY294002 prevents fMLF-triggered AKT phosphorylation. Total AKT is shown for comparison with β-actin as the loading control. Data shown are representative of three determinations.