Figure 4.

Class IA PI3Kα activity is required for survival after F. tularensis infection. PMNs were treated with HS-173 or GDC-0941 and then incubated for up to 24 hours in the presence and absence of LVS. (A, B) Apoptosis was assessed at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry. Data are the mean ± SEM (n=3). ns, not significant. **p<0.01, ***p<0.001, ****p<0.0001, as indicated. (C, D) Percentage of neutrophils with apoptotic nuclei at 18 hours. Data are the mean ± SEM (n=3). ns, not significant. *p<0.05, **p<0.01, ***p<0.001, as indicated. (E) HS-173 and GDC-0941 impair LVS-induced membrane translocation of p110α as judged by immunoblotting of subcellular fractions. Representative of three determinations. Memb., membranes. Cyto, cytosol.