Figure 5.

Inhibition of Class I PI3K isoforms PI3Kβ, PI3Kδ, and PI3Kγ does not prevent neutrophil apoptosis inhibition by F. tularensis. PMNs were left in medium alone or treated with IC-87114 (A) or PIK-294 (B) or TGX-221 (C) prior to further incubaction in the presence and absence of LVS. Apoptosis was quantified at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry. Data are the mean ± SEM of three independent experiments. Ns, not significant. *p<0.05, ****p<0.0001, as indicated.