Figure 6.

AKT activity is not required for neutrophil survival. (A, B) Neutrophils were treated with MK-2206 for 30 minutes (A) or Afuresertib for 60 minutes (B) prior to further incubation in the presence or absence of LVS. Apoptosis was assessed at 4, 10, 18, and 24 hours using Annexin V-FITC/PI staining and flow cytometry. Data are the mean ± SEM of three independent experiments. ns, not significant. *p<0.05, **p<0.01, ***p<0.001, ****p<0.001, as indicated. (C, D) Immunoblots of cell lysates were probed to detect phosphorylated AKT (P-AKT) with β-actin as the loading control. (C) Extent of AKT phosphorylation at 0, 3, 6, 12 and 24 hours in uninfected and infected cells, as indicated. (D) Effects of Afuresertib and MK-2206 on AKT phosphorylation in LVS-infected cells. Data shown are representative of three determinations.