FIGURE 4.

Additional targets repressed by GcvB through both R1 and R3 or exclusively by R3. Base‐pairing interactions of (a) cstA, (b) sucC, (c) map, and (d) ydeE were predicted by IntaRNA program. GFP reporter assays of the translational fusions in overnight‐grown Escherichia coli ΔgcvBΔsroC strain harboring pTP11 (vector), pP_L‐gcvB (GcvB), or its derivatives. Mean fluorescence of biological replicates (n > 3) with SD are presented in percentage relative to the vector control. Statistical significance was calculated using one‐way ANOVA comparing GcvB and its derivatives with the vector control and denoted as follows: ***p < .001, **p < .005, *p < .05