TABLE 3.
DIG-labeled DNA probes hybridizing to the tl gene of V. parahaemolyticus.
| Probe | Primer | Sequences for PCR | Product size (bp) | Target for hybridizationa |
|---|---|---|---|---|
| VPHP | Forward (VPF1) | 5′-TGATCAGCACGCAAGAAAAC-3′ | 922 | Nucleotides 104 to 1025 |
| Reverse (VPR1) | 5′-GTTAGCGTCTCGAACAAGGC-3′ | |||
| VP2P | Forward (VP2F1) | 5′-ATACTCACGCCTTGTTCG-3′ | 252 | Nucleotides 998 to 1249 |
| Reverse (VP2R1) | 5′-GGTACTCGGCTAAGTTGTTG-3′ |
a
Nucleotide positions are relative to the first nucleotide of the translational start codon of the tl gene of V. parahaemolyticus (GenBank accession no. M36437), which contains an ORF of 1,254 nucleotides (excluding the stop codon).