Figure 1. CoNPs exposure induces lethality in C. elegans.

WT L1 C. elegans nematodes were exposed to 0, 25, 50, and 75 μg/mL of CoNPs for 12 h in the absence of food, as described in Basic Protocol 1. The nematodes were then scored for lethality, as described in Basic Protocol 2. The experiment was performed with 3 technical replicates, with 40 animals per plate. The experiments were independently repeated twice. The data are presented in a box plot showing % of survival, compared to the control. * P < 0.05 and ** P < 0.01 compared with control group using one way ANOVA and Dunnett’s multiple comparisons test.