Figure 3. CoNPs impairs locomotion in C. elegans.

WT L1 C. elegans nematodes were exposed to 0, 25, 50, and 75 μg/mL of CoNPs for 12 h in the absence of food. Locomotion behavior was then analyzed. (A) head thrash and (B) body bend numbers were both impaired in response to CoNPs, compared to control. 40 animals were analyzed per group. ** P < 0.01 and *** P < 0.001 compared with control group using one way ANOVA and Dunnett’s multiple comparisons test.