Skip to main content
. 2022 Jul 20;8(29):eabm7833. doi: 10.1126/sciadv.abm7833

Fig. 3. Multiepitope nanofibers generate strong cellular responses against Trp2 and Td epitopes.

Fig. 3.

Mice were subcutaneously immunized with indicated formulations on days 0 and 14, and splenocytes from immunized mice were harvested for IFNγ-specific ELISPOT. Both P/Tr/Td-fiber and Tr/Td-fiber generated strong IFNγ-secreting cellular responses upon stimulation with Trp2 peptides (A) and Td peptides (B) (*P < 0.05, **P < 0.01, and ****P < 0.0001, multiple comparison by one-way ANOVA with Dunnett test, as compared to PBS group, N = 10). (C) Mice were immunized with indicated formulations on days 0, 14, and 28. PBMCs from immunized mice were sampled on days 7, 21, 35, and 56 for the presence of Trp2-specific CD8+ T cells. Left, flow histograms; right, data summary. No statistical differences were found between different immunization groups. (****P < 0.0001 between day 35 and all other days within each immunization group by multiple comparison using two-way ANOVA with Tukey test, N = 5).