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. 2022 Jul 20;8(29):eabk3150. doi: 10.1126/sciadv.abk3150

Fig. 3. Prophylactic immunization of C57BL/6J mice with varying doses of Ad5-TRP2.

Fig. 3.

Mice (n = 5 per group) were vaccinated subcutaneously with the indicated dose of Ad5-TRP2 and then (A) assessed for levels of TRP2-specific CD3+CD8+ T lymphocytes (on day 14 after vaccination) using direct immunostaining (see Materials and Methods) and flow cytometry (expressed as a percentage of total CD3+CD8+ T lymphocytes). Error bars represent SD. P values were determined by one-way ANOVA with Tukey posttest. On day 14 after vaccination, all mice were challenged with B16.F10 cells, and (B) survival was recorded and a survival graph generated using Prism software. *P < 0.05 (for all treated groups compared to the naïve group) as determined by the log-rank test adjusted for multiple comparisons.