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. 2022 Jul 20;12(7):e989. doi: 10.1002/ctm2.989

FIGURE 2.

FIGURE 2

GW4968 and Rab27a silencing down‐regulate miR‐20a in HCC827and H1299 cells. (A) Following co‐culture with cancer‐associated fibroblast (CAF)‐derived exosomes treated with GW4869 or shRab27a, miR‐20a expression was analyzed in HCC827 and H1299 cells. (B) PKH67‐labelled exosomal uptake by the treated HCC827 and H1299 cells, with representative images being shown. (C) miR‐20a levels were measured via qPCR in CAFs following miR‐20a mimic or inhibitor treatment. ***p < .001 versus Control. (D) Relative expression of miR‐20a was assessed via qPCR in exosomes derived from CAFs, which were transfected with miR‐20a mimic or inhibitor. (E) After transfection with miR‐20a mimic or inhibitor in CAFs, CAF‐derived exosomes were collected and used to treat HCC827 or H1299 cells, with miR‐20a expression in these cells then being assessed via qPCR. ***p < .001 versus Control