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. 2022 Jul 20;12(7):e989. doi: 10.1002/ctm2.989

FIGURE 6.

FIGURE 6

Overexpression of PTEN suppresses non‐small‐cell lung cancer (NSCLC) cell chemoresistance, proliferation and apoptotic resistance. PTEN was knocked down or overexpressed in H1299 and HCC8237 cells. (A and B) PTEN expression in these cells was examined via qPCR and Western blotting. Cellular viability (C) and DDP IC50 values (D) were measured. (E) The proliferation of these cells was monitored via EdU uptake assay in the absence (left) or presence of DDP (2 μg/ml; Right). (F) The proliferation of these cells was monitored via colony formation assay in the absence (left) or presence of DDP (2 μg/ml; Right). (G) The apoptotic ratio values for these cells were measured via Hoechst staining in the absence (left) or presence of DDP (20 μM; Right). (H and I) PI3K‐p85, PI3K‐p100, PI3K, pAKT and AKT levels were measured via Western blotting the indicated cells in the presence or absence of DDP treatment. GAPDH employed as a normalization control. Outcomes are means ± standard deviation (SD) (*p < .05; **p < .01; ***p < .001)