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. 2022 Jul 20;12(7):e989. doi: 10.1002/ctm2.989

FIGURE 8.

FIGURE 8

miR‐20a‐containing exosomes derived from cancer‐associated fibroblasts (CAFs) regulate the PI3K/AKT pathway in vivo to promote non‐small‐cell lung cancer (NSCLC) tumour growth and chemoresistance. Nude mice were subcutaneously implanted with HCC827 cells treated using PBS, normal tissue‐associated fibroblasts (NAFs), CAF or CAFs‐shRab27a. Mice were then randomized into DDP treatment and untreated control groups. (A) Images of representative xenograft tumours. (B) Tumour volumes were analysed once weekly beginning 1 week after implantation. (C) The expression of miR‐20a was assessed via qPCR in tumours from untreated mice. (D) The expression of miR‐20a was assessed via qPCR in tumours from DDP‐treated mice. (E) PTEN, PI3K‐p85, PI3K‐p100, PI3K, pAKT and AKT were analysed via Western blotting in tumour‐bearing mice. (F) Tumours from xenograft model mice were subjected to Ki‐67 and TUNEL staining to analyse apoptotic cell death (200x), with the positive staining area being quantified in (G and H). Results are representative data from triplicate experiments, and outcomes are means ± standard deviation (SD) (*p < .05; **p < .01; ***p < .001)