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. 2021 Nov 10;150(4):688–704. doi: 10.1002/ijc.33859

FIGURE 2.

FIGURE 2

Synchronous activation of naïve OT‐I T cells demonstrates early induction of PD‐1, but late expression of NKG2A. (A) Schematic overview of this in vitro activation system for naïve CD8 T cells from OT‐I mice by coculture with adherent SAMBOK cells, fibroblasts transfected with B7‐1 (CD80) and the minigene encoding SigOVA257‐264. OT‐I T cells were overnight stimulated on these engineered presenting cells and then transferred to fresh wells with the homeostatic cytokine IL‐7, preventing chronic T cell receptor triggering. (B) Representative flow cytometry plots from three independent experiments displaying activation markers CD62L and CD44 on OT‐I T cells after the first stimulation by SAMBOK. (C) Surface expression of PD‐1 and NKG2A on OT‐I T cells after different stimulation schemes with SAMBOK at the indicated timepoints (arrow). One representative out of seven independent experiments is shown [Color figure can be viewed at wileyonlinelibrary.com]