Figure 1.

Short telomeres in bur1 and bur2 mutants. (a) Southern blot analysis of telomeres from WT, bur2∆, bur1–1, and bur1–8 mutants. bur2∆ isolated from the haploid screen of yeast deletion mutants is indicated with an arrow (Lane 5), in addition two independent segregants of bur2∆ haploids from BUR2/bur2∆ heterozygous diploids are shown (Lanes 3, 4). Telomere lengths of bur1–1 and bur1–8 temperature‐sensitive alleles of BUR1 grown at 25°C and 30°C, respectively, are shorter than WT (Lanes 7, 8 and 9, 10). (b) The short telomere length in bur1–8 is rescued by a centric plasmid expressing BUR1 (Lanes 5, 6). (c) Telomere length in bur1–8 and bur1–8 set2∆ mutants is shorter than WT and set2∆ (Lanes 5, 6). (d) Lethality of bur1∆ is rescued by a plasmid expressing histone mutant H3:K36A; however, telomere length is shorter than WT (Lanes 3, 4, 5). (e) Growth rate of bur1–8, set2∆, bur1–8 set2∆ mutants at permissive (25°C) and restrictive (37°C) temperature. Serial 10‐fold dilution of cells plated at different temperatures indicated bur1–8 set2∆ mutants grow better than bur1–8 mutants alone at 37°C