Figure 4.

Overexpression of Tlc1 does not rescue de novo telomere elongation. (a) De novo telomere elongation in bur2∆ set2∆ cells with or without pGalTLC1. Two independent isolates of bur2∆ set2∆ were examined and compared to WT cells or set2∆ mutants alone. (b) qRT‐PCR of EST1, 2, 3, and TLC1 and the control ARN1 from RNA isolated during the de novo elongation assay at T = 4 h shown in part (a). Tlc1 is the only component downregulated in bur2∆ set2∆ cells, and the levels are increased over 10‐fold in cells containing the pGalTLC1 plasmid. Error bars represent SEM (N = 3 technical replicates [Colour figure can be viewed at wileyonlinelibrary.com]