Figure 5.

Overexpression of Tlc1 does not rescue telomere length in bur1∆ set2∆ cells. Telomere length was not increased by pGalTlc1 overexpression in bur1∆ set2∆ cells (Lanes 10, 11, 12, 13). Two of the haploids from independent diploid dissections are the same strains used for de novo telomere addition in Figure 4. The other two haploids are isogenic from the same dissections. These selectively grown haploids were propagated after the second passage in CAA media containing galactose and no tryptophan for plasmid selection. Cultures were harvested after two additional passages for analysis, for a total of four passages. Shortening in cells containing the overexpressed Tlc1 RNA is most likely due to titration of the Ku proteins from telomeres