Figure 8.

Survivors are not observed in bur1–8 tlc1∆ double mutants. Successive passages of bur1–8, tlc1∆, and bur1–8 tlc1∆ double mutants at either 30°C or 34°C. Cells were kept in continuous daily culture and DNA prepared at Day 2, 6, or 7. Survivors were generated at Days 6 and 7 in the tlc1∆ strains at both temperatures, as indicated by the higher molecular weight telomere bands in this Southern analysis (Lanes 13, 14 and 23, 24). Survivors were not seen in the bur1–8 tlc1∆ double mutants at this time period (Lanes 10, 11 and 20, 21)