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. 2021 Dec 2;39(3):177–192. doi: 10.1002/yea.3680

Table 2.

Plasmids used in this study

Plasmid name Brief description Source
pRS316 Control plasmid for experiments Sikorski & Hieter, 1989
pBUR1 Contains BUR1 coding region in pRS316 (GP111, JHU1169) G. Prelich gift
pRS426BUR1 Contains BUR1 coding region in pRS426 (JHU1171) This study
pBUR1∆C Contains BUR1 1–365aa only in pRS316 (JHU1255) This study
pBS4 (pH3:K36A) Plasmid in EMHy201 (HHT2:K36A HHF2/TRP/CEN/ARS/AmpR) Hyland et al., 2005
pESC‐TRP‐TLC1/HAT EST2 Overexpression plasmid containing TLC1 and EST2 (JHU1054) V. Zakian gift
pGal1TLC1 Overexpression plasmid containing TLC1 derived from pesc‐TRP‐TLC1/HAT EST2 (JHU1238) This study
SCR1/topo PCR2.1 Control plasmid for SCR1 expression (BCC41) This study
ARN1/topo PCR2.1 Control plasmid for ARN1 expression (BCC43) This study
pVL1091 (JHU995) Control plasmid for EST1 expression (Cdc13‐Est1 fusion/pRS415) Evans & Lundblad, 1999
pVL369 (JHU612) Control plasmid for EST2 expression (2μ ADH‐EST2 integrating) Lingner et al., 1997
pAY30 (JHU1166) Control plasmid for TLC1 expression (pRS316 TLC1) A. IJpma collection