Table 3.
Primers used in this study
| Primer ID | 5′‐3′ sequence | Function | Source |
|---|---|---|---|
| OCC8 | CGAATATTTAGAGAGAATCCGTCAC | A primer for YLR226W BUR2 | Winzeler et al., 1999 |
| OCC16 | TCAGTTATGGCTGTAGGTATTCCAT | B primer for YLR226W BUR2 | Winzeler et al., 1999 |
| OCC162 | TTTTGAATCATATTGAAACAAGGGT | C primer for YLR226W BUR2 | Winzeler et al., 1999 |
| OCC163 | TCGAAAATATTATTGATGCTTGTGA | D primer for YLR226W BUR2 | Winzeler et al., 1999 |
| OCC164 | CGTAGTATTTTCGTTTAAAATATATTACAGTAAGATAATGAGATTGTACTGAGAGTGCAC | Upstream primer for KO of YLR226W BUR2 | Winzeler et al., 1999 |
| OCC165 | CTGATCCCTCCAATTAAACATAACTTGTACTCTATTTTTACTGTGCGGTATTTCACACCG | Downstream primer for KO of YLR266W BUR2 | Winzeler et al., 1999 |
| OCC166 | GACCTAGGTCTCATTGTGACT | A1 primer for YLR226W BUR2, 170 bp from ATG | This study |
| OCC167 | AAGGTACTGTTGACTGCTAT | D1 primer for YLR226W BUR2, 304 bp down from TAA | This study |
| OCC176 | GGTAGCAACTCTGATATTCCACTGT | A confirmation primer for YPR161C BUR1, 258 bp up from ATG | Winzeler et al., 1999 |
| OCC177 | CTGTACACCCGTAAACTTTCTCACT | B confirmation primer for YPR161C BUR1 | Winzeler et al., 1999 |
| OCC178 | AGGAGTTAATAGATACGGACCCAAC | C confirmation primer for YPR161C BUR1 | Winzeler et al., 1999 |
| OCC179 | TTTTTGGCACTCTTTTAAATGGTAT | D confirmation primer for YPR161C BUR1 | Winzeler et al., 1999 |
| OCC182 | CAGATGCAGATCATTCTTCAGGAAT | A1 confirmation primer for YPR161C BUR1 | This study |
| OCC183 | TTGAACCAGTGACTTAGCTGGGAGT | D1 confirmation primer for YPR161C BUR1 426 bp down from TAA | This study |
| OCC224 | GAGAAGAAGCTGACTTCGACTATTG | A confirmation primer for YJL168C SET2 | Winzeler et al., 1999 |
| OCC174 | TTCAGTATTTCTTTTTCATCTTCCG | B confirmation primer for YJL168C SET2 | Winzeler et al., 1999 |
| OCC175 | GCTAAAGACATCGTGAAAATCCTAA | C confirmation primer for YJL168C SET2 | Winzeler et al., 1999 |
| OCC225 | AAAAATAAAGACACTTGAAACGCAC | D confirmation primer for YJL168C SET2 | Winzeler et al., 1999 |
| OCC228 | GTGGGATGGGATACGTTGAG | SCR1(pol lll transcript) forward primer. Anneal to nt 20–39 (YM) | This study |
| OCC229 | TTTACGACGGAGGAAAGACG | SCR1(pol lll transcript) reverse primer. Anneals to nt 144–163 (YM) | This study |
| OCC230 | ACCGATCCTCTTCTCGACCT | TLC1 forward primer. Anneals to nt 417–436 (YM) | This study |
| OCC231 | TAAACAGCGAACTCGTGCAA | TLC1 reverse primer. Anneals to nt 516–535 (YM) | This study |
| OCC232 | GAGATGAACAACAGCGCAAA | EST1_F forward primer (SVC) | This study |
| OCC233 | GAAACGCCATCTTTTTCTGG | EST1_R reverse primer (SVC) | This study |
| OCC234 | AAAACTGGCTGACGATTTCC | EST2_F forward primer (SVC) | This study |
| OCC235 | TTGGGAGCTTACGGCTAAAA | EST2_R reverse primer (SVC) | This study |
| OCC236 | ATCATTGGCGATGCTGACTT | EST3_F forward primer (SVC) | This study |
| OCC237 | CAAATATCGTGGCCTGGTTT | EST3_R reverse primer (SVC) | This study |
| OCC238 | AATGGAGGGCCAGAAAGACT | ARN_F forward primer (SVC) | This study |
| OCC239 | TCAAAAGGACACCAACGACA | ARN_R reverse primer (SVC) | This study |
| OCC248 | TGACGGGCGAATTATACAAC | EST2_F new forward primer | This study |
| OCC249 | ATTTTCCAAGCAGCGCCTTT | EST2_R new reverse primer | This study |
| OCC274 | atccttggtttaaagaggactagGTTATACTATTCTCTCTTCCTTTCTGGC | GP111fwd Gibson deletion of BUR1 for BUR1∆C | This study |
| 0CC275 | AGAGAGAATAGTATAACctagtcctctttaaaccaaggatggtgt | GP111rev Gibson deletion of BUR1 for BUR1∆C | This study |