Table 2. Live-cell fluorescence resonance energy transfer (FRET) max normalization experiment data and statistics.
Table 2—source data 1. Source data for Table 2.
elife-78982-table2-data1.xlsx (20.2KB, xlsx)
| Sensor | Ligand | N | Mean max response | SEM |
|---|---|---|---|---|
| SNAP-m2 | Glutamate | - | 1 | - |
| SNAP-m2 | DCG-IV | 25 | 0.79 | 0.01 |
| SNAP-m2 | LY379268 | 23 | 1.01 | 0.01 |
| SNAP-m2 | (2R,4R)-APDC | 14 | 0.96 | 0.01 |
| SNAP-m2 | Glutamate + 10 μM BINA | 7 | 1.02 | 0.01 |
| SNAP-m2 | Glutamate + 5 μM LY487379 | 14 | 1.07 | 0.01 |
| SNAP-m2 | Glutamate + 0.5 μM JNJ-42153605 | 22 | 1.01 | 0.01 |
| SNAP-m2 | Glutamate + 10 μM MNI-137 | 22 | 0.85 | 0.02 |
| SNAP-m2 | Glutamate + 10 μM Ro 64–5229 | 35 | 0.87 | 0.01 |
| azi-CRD | Glutamate | - | 1 | - |
| azi-CRD | DCG-IV | 19 | 0.69 | 0.01 |
| azi-CRD | LY379268 | 25 | 1.06 | 0.02 |
| azi-CRD | (2R,4R)-APDC | 13 | 1.02 | 0.01 |
| azi-CRD | Glutamate + 10 μM BINA | 9 | 1.12 | 0.05 |
| azi-CRD | Glutamate + 5 μM LY487379 | 19 | 1.56 | 0.07 |
| azi-CRD | Glutamate + 0.5 μM JNJ-42153605 | 8 | 1.43 | 0.08 |
| azi-CRD | Glutamate + 10 μM MNI-137 | 18 | 0.86 | 0.02 |
| azi-CRD | Glutamate + 10 μM Ro 64–5229 | 18 | 0.59 | 0.03 |
| azi-ECL2 | Glutamate | - | 1 | - |
| azi-ECL2 | DCG-IV | 25 | 0.64 | 0.02 |
| azi-ECL2 | LY379268 | 22 | 1.14 | 0.04 |
| azi-ECL2 | (2R,4R)-APDC | 56 | 1.05 | 0.01 |
| azi-ECL2 | Glutamate + 10 μM BINA | 14 | 1.42 | 0.07 |
| azi-ECL2 | Glutamate + 5 μM LY487379 | 7 | 1.25 | 0.09 |
| azi-ECL2 | Glutamate + 0.5 μM JNJ-42153605 | 13 | 0.99 | 0.02 |
| azi-ECL2 | Glutamate + 10 μM MNI-137 | 58 | 0.78 | 0.03 |
| azi-ECL2 | Glutamate + 10 μM Ro 64–5229 | 8 | 0.84 | 0.05 |
All max response values are normalized to 1 mM glutamate.