Figure 4. Differences in electrophysiology and contractility between cell‐seeding densities and commercial hiPSC‐CM cell lines.

A, electrophysiology of hiPSC‐CMs showing spontaneous beating frequency (Aa) T_Rise (Ab) and APD₉₀ (Ac) for both NCardia (black) and ICell² (grey) hiPSC‐CM cell lines. B, contractile behaviour of hiPSC‐CMs shown as contraction time (Ba), relaxation time (Bb) and CD₅₀ (Bc) for both Cor.4U (dark grey bars) and ICell² (light grey bars) hiPSC‐CM cell lines. n = 5–19 (ICell²) and 9–30 (Cor.4U), N = 3 experiments for both cell lines. Cell densities were compared with Ctrl for each cell type using mixed effect two‐way ANOVA with Dunnett's post hoc test (statistical significance indicated with ˆ). Differences between NCardia and ICell² were tested between a two‐way ANOVA with Sidak's post hoc test (statistical significance indicated with ^*). Recordings were made on day 2 after stencil removal. Absolute values of the P values for all comparisons (significant and non‐significant) are listed in the online Statistical Supplement.