Figure 2.
Flowchart of the patients included and the assays and analyses performed. Patients were excluded from analysis if: *PD‐L1 IHC was not evaluable due to too few or no viable tumor cells left in tissue; #multiplex IHC (IHC of CD8, FOXP3, PD‐1, and pan‐cytokeratin) was not evaluable due to failed pan‐cytokeratin IHC, failed cell detection in image analysis, no tumor tissue left, or failed digital scan; $no matching biopsy or resection was available for analysis. nCRT, neoadjuvant chemoradiation; S, surgery.