TABLE 1.
Relative changes in gene expression (fold) after 3 weeks of hAC encapsulated in gelMA or pellet culture compared to day 0 (= time point of encapsulation) for the chosen Donor 3 P3
| gelMA concentration/culture model | TGF‐β3 concentration | COL2 | COL1 | Differentiation index (COL2/COl1) | ACAN | VCAN | Differentiation index (ACAN/VCAN) |
|---|---|---|---|---|---|---|---|
| 7.5% | 0 ng/mL | 2.4 | 0.8 | 3.5 | 0.7 | 0.7 | 0.6 |
| 1 ng/mL | 1.9 × 103 | 10.9 | 225.9 | 2.9 | 0.7 | 3.9 | |
| 10 ng/mL | 1.9 × 105 | 20.5 | 11.4 × 103 | 64.1 | 3.1 | 19.3 | |
| 10% | 0 ng/mL | 1.3 | 0.5 | 2.4 | 0.4 | 0.6 | 0.8 |
| 1 ng/mL | 2.8 × 103 | 14.1 | 212.1 | 8.1 | 1.2 | 6.8 | |
| 10 ng/mL | 2.1 × 105 | 39.0 | 5.5 × 103 | 128.8 | 4.2 | 31.3 | |
| Pellet culture | 0 ng/mL | 131.5 | 0,3 | 426.1 | 1.3 | 0.5 | 2.6 |
| 1 ng/mL | 1.9 × 105 | 32.8 | 6.3 × 103 | 63.2 | 2.0 | 34.0 | |
| 10 ng/mL | 0.9 × 105 | 34.5 | 2.3 × 103 | 53.2 | 3.0 | 17.1 |
Abbreviations: ACAN, aggrecan; COL1, collagen type I; COL2, collagen type II; gelMA, Gelatin methacryloyl; hAC, human articular chondrocytes; VCAN, versican.