Fig. 6.
Overexpression of CsiLAC4 (CsiLAC4OE), rather than of CsiLAC17 (CsiLAC17OE) and pre‐miR397 (miR397OE) in Arabidopsis, decreases free boron (B) concentrations under excess boric acid treatments. B fractions in the root (a–c) and shoots (d–f) of 14‐d‐old seedlings, roots (g–i) and leaves (j–l) from mature plants were extracted as described by Martinez‐Cuenca et al. (2015). All fractions were finally determined by inductively coupled plasma‐MS. Free B in roots (a, d, g, j). Cell wall (CW)‐bound B (b, e, h, k). Organic‐bound B (c, f, i, l). Results are mean ± SD. Asterisks indicate significant differences at: *, P < 0.05; and **, P < 0.01 by one‐way ANOVA (Student‐Newman‐Keuls method, n = 4). FW, fresh weight; DW, dry weight.