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. 2021 Dec 11;75(4):814–830. doi: 10.1002/hep.32166

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© 2021 The Authors. Hepatology published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Modifications of the peribiliary vascular plexus. (A) IHC for vWF in bile duct specimens obtained from patients retransplanted for nonbiliary causes (control), NAS, or HAT. vWF⁺ vessels (arrows) and the MVD around PBGs (arrowheads) are shown. Areas in circles are magnified below. Scale bars, 200 µm. (B) H&E stains show normal vessels (arrows) and damaged ones (arrowheads) in the bile duct wall. Areas in squares are magnified below. Original magnification: ×20. (C) TUNEL assay shows nonapoptotic (blue arrows) and apoptotic (brown arrows) endothelial cells (ECs). Original magnification: ×40. (D) Histograms show quantification (mean and SD) of vWF⁺ microvessel (MV) density within bile ducts, score of EC disruption, and TUNEL⁺ ECs. *p < 0.05 versus other groups