Table 3.
Performance overview of methods for gene library assembly (e. g. for testing various protein designs).
|
Method |
Gene size (bp) |
Pool size[a] |
% coverage[b] |
% accurate assemblies[c] |
Ref. |
|---|---|---|---|---|---|
|
MPA |
192–252 |
131 to 250 |
72.7 to 96.4 |
11.8 to 31.3 |
[38] |
|
192–252 |
1212 |
84.2 |
11.8 to 31.3 |
[38] |
|
|
192–252 |
2271 |
70.6 |
11.8 to 31.3 |
[38] |
|
|
DropSynth 2.0 |
675 |
384 |
92.0 |
23.5 |
[43] |
|
675 |
1536 |
80.0 |
22.6 to 27.6 |
[43] |
[a] Number of independent assemblies performed in one pool. [b] Number of assemblies (per 100 designed assemblies) that show at least one error‐free molecule. [c] Number of error‐free assembled molecules per 100 assembled molecules.