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. Author manuscript; available in PMC: 2022 Dec 2.
Published in final edited form as: Nat Biomed Eng. 2021 Dec 2;5(12):1500–1516. doi: 10.1038/s41551-021-00823-9

Extended Fig. 14: H3K9me3 effects gene expression globally, but not locally.

Extended Fig. 14:

a) RNAseq data of CMs from H3K9M and H3WT was analyzed with respect to their distance to H3K9me3 heterochromatin domains, using a ChIPseq dataset of 16.5-day mouse embryo hearts from modEncode. Left: The combined expression of H3K9M and H3WT showed a general decrease of gene expression with proximity to heterochromatin domains. Right: Relative expression in H3K9me3 suppressed H3K9M mice, compared to H3WT, showed no direction of expression change, only higher variance. b) Example of a single gene track for Nkx2.5. Repressive polycomb domains are shown in purple (ReprPC). Although there is a repressive polycomb domain near the TSS of this gene of interest there is no significant upregulation of the level of gene expression according to our RNA-seq data.