Extended Table 1. Expression of histone variants in cardiac cultures on cultured on soft or stiff substrates.
Embryonic cardiac cells were plated on soft (13 kPa) or stiff (140 kPa) PDMS for four days after which total RNA was harvested to perform RNAseq analysis. Averaged expression (FPKM: Fragments Per Kilobase of transcript per Million mapped reads) for each substrate group, as well as fold changes and p-values between substrates, of previously annotated mouse histone variants91 are shown; * indicates canonical (generic) H1 variants.
| Gene | Ensembl ID | Family | Variant | FPKM 13 kPa | FPKM 140 kPa | Fold Change | p-Value |
|---|---|---|---|---|---|---|---|
| Hist1h1a | ENSMUSG00000049539 | H1 | H1.1* | 32.92 | 22.83 | 0.694 | 0.595 |
| Hist1h1c | ENSMUSG00000036181 | H1 | H1.2* | 75.57 | 55.23 | 0.731 | 0.413 |
| Hist1h1d | ENSMUSG00000052565 | H1 | H1.3* | 65.25 | 35.79 | 0.549 | 0.350 |
| Hist1h1e | ENSMUSG00000051627 | H1 | H1.4* | 66.10 | 43.53 | 0.658 | 0.386 |
| Hist1h1b | ENSMUSG00000058773 | H1 | H1.5* | 41.15 | 26.51 | 0.644 | 0.531 |
| H1f0 | ENSMUSG00000096210 | H1 | H1.0 | 60.85 | 51.81 | 0.851 | 0.015 |
| H1fx | ENSMUSG00000044927 | H1 | H1.X | 0.14 | 0.01 | 0.088 | 0.239 |
| Gm6970 | ENSMUSG00000091230 | H1 | H1.11 | 0.43 | 0.06 | 0.150 | 0.306 |
| Hist1h1t | ENSMUSG00000036211 | H1 | TS H1.6 | 0 | 0.80 | - | - |
| H1fnt | ENSMUSG00000048077 | H1 | TS H1.7 | 0 | 0 | - | - |
| H1foo | ENSMUSG00000042279 | H1 | OO H1.8 | 0 | 0 | - | - |
| Hils1 | ENSMUSG00000038994 | H1 | TS H1.9 | 0 | 0 | - | - |
| Hist1h2ab | ENSMUSG00000061615 | H2A | canonical | 14.08 | 3.38 | 0.240 | 0.335 |
| Hist1h2ac | ENSMUSG00000069270 | H2A | canonical | 7.33 | 1.20 | 0.163 | 0.286 |
| Hist1h2ad | ENSMUSG00000071478 | H2A | canonical | 5.11 | 1.05 | 0.205 | 0.370 |
| Hist1h2ae | ENSMUSG00000069272 | H2A | canonical | 4.83 | 0.77 | 0.160 | 0.314 |
| Hist1h2ag | ENSMUSG00000069301 | H2A | canonical | 6.01 | 1.27 | 0.211 | 0.379 |
| Hist1h2ai | ENSMUSG00000071516 | H2A | canonical | 5.27 | 0.80 | 0.153 | 0.312 |
| Hist1h2an | ENSMUSG00000069309 | H2A | canonical | 3.65 | 0.41 | 0.112 | 0.330 |
| Hist1h2ao | ENSMUSG00000094248 | H2A | canonical | 6.25 | 1.15 | 0.184 | 0.356 |
| Hist1h2ap | ENSMUSG00000094777 | H2A | canonical | 6.26 | 1.05 | 0.169 | 0.351 |
| Hist1h2af | ENSMUSG00000061991 | H2A | canonical | 3.94 | 0.92 | 0.234 | 0.286 |
| Hist1h2ah | ENSMUSG00000069302 | H2A | canonical | 4.99 | 0.78 | 0.156 | 0.314 |
| Hist1h2ak | ENSMUSG00000063021 | H2A | canonical | 14.40 | 1.52 | 0.106 | 0.274 |
| Hist1h2al | ENSMUSG00000091383 | H2A | canonical | 0.38 | 0.07 | 0.194 | 0.143 |
| Hist2h2aa1 | ENSMUSG00000064220 | H2A | canonical | 37.72 | 10.72 | 0.284 | 0.314 |
| Hist2h2aa2 | ENSMUSG00000063954 | H2A | canonical | 44.72 | 13.15 | 0.294 | 0.322 |
| Hist2h2ab | ENSMUSG00000063689 | H2A | canonical | 1.31 | 0.19 | 0.144 | 0.212 |
| Hist2h2ac | ENSMUSG00000068855 | H2A | canonical | 16.41 | 4.61 | 0.281 | 0.243 |
| Hist3h2a | ENSMUSG00000078851 | H2A | canonical | 8.28 | 5.36 | 0.648 | 0.151 |
| H2afj | ENSMUSG00000060032 | H2A | H2A.J | 3.03 | 2.30 | 0.761 | 0.445 |
| H2afx | ENSMUSG00000049932 | H2A | H2A.X | 8.63 | 4.77 | 0.553 | 0.416 |
| H2afz | ENSMUSG00000037894 | H2A | H2A.Z | 69.22 | 75.76 | 1.094 | 0.638 |
| H2afv | ENSMUSG00000041126 | H2A | H2A.Z | 12.45 | 11.91 | 0.956 | 0.741 |
| H2afy | ENSMUSG00000015937 | H2A | macro H2A | 22.42 | 24.38 | 1.087 | 0.603 |
| H2afy2 | ENSMUSG00000020086 | H2A | macro H2A | 9.54 | 9.11 | 0.954 | 0.735 |
| H2afy3 | ENSMUSG00000101167 | H2A | macro H2A.3 | 0 | 0 | - | - |
| Hist1h2aa | ENSMUSG00000060081 | H2A | TS H2A.1 | 0 | 0 | - | - |
| H2al1a | ENSMUSG00000100626 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1c | ENSMUSG00000096097 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1d | ENSMUSG00000094904 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1f | ENSMUSG00000095655 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1g | ENSMUSG00000095662 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1h | ENSMUSG00000099443 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1i | ENSMUSG00000095445 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1b | ENSMUSG00000101819 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1e | ENSMUSG00000095413 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1j | ENSMUSG00000069038 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1k | ENSMUSG00000100448 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1m | ENSMUSG00000100200 | H2A | H2A.L | 0.21 | 0 | - | - |
| H2al1n | ENSMUSG00000078346 | H2A | H2A.L | 0 | 0 | - | - |
| H2al1o | ENSMUSG00000061065 | H2A | H2A.L | 0 | 0 | - | - |
| H2afb1 | ENSMUSG00000062651 | H2A | H2A.L | 0 | 0 | - | - |
| H2al2b | ENSMUSG00000095573 | H2A | H2A.L | 0 | 0 | - | - |
| H2al2c | ENSMUSG00000094881 | H2A | H2A.L | 0 | 0 | - | - |
| Hypm | ENSMUSG00000040456 | H2A | H2A.P | 0 | 0 | - | - |
| H2afb2 | ENSMUSG00000082482 | H2A | H2A.B | 0 | 0 | - | - |
| H2afb3 | ENSMUSG00000083616 | H2A | H2A.B | 0 | 0 | - | - |
| Gm14920 | ENSMUSG00000067441 | H2A | H2A.B | 0 | 0 | - | - |
| Hist1h2bb | ENSMUSG00000075031 | H2B | canonical | 58.66 | 31.09 | 0.530 | 0.330 |
| Hist1h2bc | ENSMUSG00000018102 | H2B | canonical | 75.02 | 62.26 | 0.830 | 0.547 |
| Hist1h2be | ENSMUSG00000047246 | H2B | canonical | 8.78 | 4.93 | 0.561 | 0.402 |
| Hist1h2bg | ENSMUSG00000058385 | H2B | canonical | 54.19 | 29.79 | 0.550 | 0.522 |
| Hist1h2bf | ENSMUSG00000069268 | H2B | canonical | 12.99 | 7.59 | 0.584 | 0.482 |
| Hist1h2bj | ENSMUSG00000069300 | H2B | canonical | 31.22 | 10.63 | 0.341 | 0.386 |
| Hist1h2bl | ENSMUSG00000094338 | H2B | canonical | 25.52 | 14.71 | 0.577 | 0.506 |
| Hist1h2bn | ENSMUSG00000095217 | H2B | canonical | 58.33 | 31.99 | 0.548 | 0.460 |
| Hist1h2bq | ENSMUSG00000069307 | H2B | canonical | 6.30 | 3.45 | 0.548 | 0.404 |
| Hist1h2br | ENSMUSG00000069303 | H2B | canonical | 11.13 | 5.64 | 0.507 | 0.343 |
| Hist1h2bq | ENSMUSG00000069307 | H2B | canonical | 6.30 | 3.45 | 0.548 | 0.404 |
| Hist1h2br | ENSMUSG00000069303 | H2B | canonical | 11.13 | 5.64 | 0.507 | 0.343 |
| Hist1h2bh | ENSMUSG00000064168 | H2B | canonical | 21.22 | 9.45 | 0.445 | 0.396 |
| Hist1h2bk | ENSMUSG00000062727 | H2B | canonical | 44.56 | 20.03 | 0.450 | 0.345 |
| Hist1h2bm | ENSMUSG00000096807 | H2B | canonical | 32.08 | 19.90 | 0.620 | 0.483 |
| Hist1h2bp | ENSMUSG00000069308 | H2B | canonical | 7.24 | 3.81 | 0.526 | 0.264 |
| Hist2h2be | ENSMUSG00000068854 | H2B | canonical | 0.83 | 2.17 | 2.617 | 0.019 |
| Hist3h2ba | ENSMUSG00000056895 | H2B | canonical | 1.47 | 0.95 | 0.647 | 0.615 |
| Hist3h2bb | ENSMUSG00000080712 | H2B | canonical | 1.66 | 2.26 | 1.357 | 0.517 |
| Hist1h2ba | ENSMUSG00000050799 | H2B | sperm H2B | 0.72 | 0.47 | 0.647 | 0.489 |
| Hist2h2bb | ENSMUSG00000105827 | H2B | canonical | 0 | 0 | - | - |
| 1700024p04rik | ENSMUSG00000045022 | H2B | subH2B | 0 | 0 | - | - |
| H2bfm | ENSMUSG00000048155 | H2B | H2B.W | 0 | 0 | - | - |
| Hist1h3a | ENSMUSG00000069265 | H3 | canonical | 13.94 | 5.18 | 0.372 | 0.384 |
| Hist1h3g | ENSMUSG00000099517 | H3 | canonical | 16.82 | 5.27 | 0.313 | 0.279 |
| Hist1h3h | ENSMUSG00000101355 | H3 | canonical | 21.59 | 6.20 | 0.287 | 0.226 |
| Hist1h3i | ENSMUSG00000101972 | H3 | canonical | 39.53 | 23.30 | 0.589 | 0.537 |
| Hist1h3b | ENSMUSG00000069267 | H3 | canonical | 24.57 | 9.76 | 0.397 | 0.341 |
| Hist1h3c | ENSMUSG00000069310 | H3 | canonical | 24.95 | 10.08 | 0.404 | 0.227 |
| Hist1h3d | ENSMUSG00000099583 | H3 | canonical | 17.60 | 7.91 | 0.449 | 0.425 |
| Hist1h3e | ENSMUSG00000069273 | H3 | canonical | 8.85 | 3.48 | 0.393 | 0.241 |
| Hist1h3f | ENSMUSG00000100210 | H3 | canonical | 11.10 | 4.05 | 0.365 | 0.307 |
| Hist2h3b | ENSMUSG00000074403 | H3 | canonical | 9.61 | 3.37 | 0.350 | 0.377 |
| Hist2h3c2 | ENSMUSG00000081058 | H3 | canonical | 3.32 | 1.37 | 0.412 | 0.401 |
| H3f3a | ENSMUSG00000060743 | H3 | H3.3 | 95.66 | 94.39 | 0.987 | 0.937 |
| H3f3b | ENSMUSG00000016559 | H3 | H3.3 | 122.89 | 130.10 | 1.059 | 0.780 |
| Gm6421 | ENSMUSG00000094518 | H3 | H3.3 | 15.72 | 10.54 | 0.670 | 0.322 |
| Gm10257 | ENSMUSG00000096789 | H3 | H3.3 | 7.37 | 5.66 | 0.767 | 0.488 |
| Cenpa | ENSMUSG00000029177 | H3 | cenH3 | 19.08 | 21.18 | 1.110 | 0.468 |
| H3f3c | ENSMUSG00000082029 | H3 | H3.5 | 116.29 | 102.04 | 0.877 | 0.287 |
| Hist2h3c1 | ENSMUSG00000093769 | H3 | canonical | 0 | 0 | - | - |
| Gm12260 | ENSMUSG00000080152 | H3 | TS H3.4 | 0 | 0 | - | - |
| Hist1h4a | ENSMUSG00000060093 | H4 | canonical | 34.85 | 11.56 | 0.332 | 0.379 |
| Hist1h4b | ENSMUSG00000069266 | H4 | canonical | 47.65 | 12.84 | 0.269 | 0.247 |
| Hist1h4c | ENSMUSG00000060678 | H4 | canonical | 102.01 | 32.57 | 0.319 | 0.291 |
| Hist1h4d | ENSMUSG00000061482 | H4 | canonical | 124.40 | 41.41 | 0.333 | 0.284 |
| Hist1h4f | ENSMUSG00000069274 | H4 | canonical | 82.80 | 37.85 | 0.457 | 0.317 |
| Hist1h4h | ENSMUSG00000060981 | H4 | canonical | 52.11 | 18.08 | 0.347 | 0.171 |
| Hist1h4i | ENSMUSG00000060639 | H4 | canonical | 4.51 | 2.49 | 0.553 | 0.234 |
| Hist1h4j | ENSMUSG00000067455 | H4 | canonical | 9.82 | 3.45 | 0.351 | 0.310 |
| Hist1h4k | ENSMUSG00000064288 | H4 | canonical | 42.69 | 21.68 | 0.508 | 0.387 |
| Hist1h4m | ENSMUSG00000069306 | H4 | canonical | 77.49 | 32.80 | 0.423 | 0.327 |
| Hist1h4n | ENSMUSG00000069305 | H4 | canonical | 65.29 | 27.97 | 0.428 | 0.340 |
| Hist2h4 | ENSMUSG00000091405 | H4 | canonical | 5.75 | 3.42 | 0.595 | 0.446 |
| Hist4h4 | ENSMUSG00000096010 | H4 | canonical | 2.34 | 0.85 | 0.363 | 0.139 |