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. 2022 Apr 14;43(28):2698–2709. doi: 10.1093/eurheartj/ehac153

Figure 2.

Figure 2

Adult T-cell transfer impairs neonatal cardiac regeneration. (A) Graphical representation of the experimental setup. Adult Thy1.1+ T-cells were transferred into Thy1.2+ neonatal (P1) WT recipients. (B) The successful transfer of Thy1.1+ cells was confirmed by flow cytometry analysis of the spleen. (C) Characterization of transferred Thy1.1+ cell subpopulation in the spleen. (D) Time course echocardiographic analysis of ejection fraction. SHAM control and SHAM + Adult T-cells n = 6, left anterior descending artery (LAD) control n = 8, LAD + adult T-cells n = 9. (E) Representative images of 2D echocardiogram in the left parasternal long-axis view. (F) Representative serial long-axis histological sections from hearts harvested 21 dpi, stained with Masson’s trichrome. The arrowheads indicate the fibrotic area. Original magnification 20×, scale bar 1000 µm (F, upper), 40×, scale bar 100 µm (F, lower). (G) Quantification of the fibrosis percentage in the left ventricle 21 dpi. P, postnatal day; dpi, days post-injury. Data are expressed as mean values ±S.D. (B, D, G). Statistical test: two-way ANOVA followed by Tukey’s multiple comparisons test (B, D, G).