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. 2022 Jun 25;39(2):119–128. doi: 10.5511/plantbiotechnology.21.1222a

Figure 2. OsCERK2 had potential function in AM symbiosis. (A–D) Trypan blue staining of OsCERK2ox;oscerk1 #9 (A), oscerk1 (B), WT (C), and OsCERK2-DVox/oscerk1 #4 (D) at 2 WAI with R. irregularis. Scale bars=100 µm. ab, arbuscles; ih, internal hypha; eh, external hypha; hp, hyphopodia. (E, F) Number of infection units/plants in WT, oscerk1 and (E) OsCERK2ox;oscerk1 #6, #9, and #12 and (F) OsCERK2-DVox/oscerk1 #4 and #10. (G, H) The expression level of (G) AM1 and (H) AM3 in WT, oscerk1, OsCERK2ox;oscerk1 #6, #9, and #12 at 2 WAI. Error bars indicate the SD from 3 replicates for WT and oscerk1 with more than 5 replicates for both OsCERK2ox;oscerk1 and OsCERK2-DVox/oscerk1.

Figure 2. OsCERK2 had potential function in AM symbiosis. (A–D) Trypan blue staining of OsCERK2ox;oscerk1 #9 (A), oscerk1 (B), WT (C), and OsCERK2-DVox/oscerk1 #4 (D) at 2 WAI with R. irregularis. Scale bars=100 µm. ab, arbuscles; ih, internal hypha; eh, external hypha; hp, hyphopodia. (E, F) Number of infection units/plants in WT, oscerk1 and (E) OsCERK2ox;oscerk1 #6, #9, and #12 and (F) OsCERK2-DVox/oscerk1 #4 and #10. (G, H) The expression level of (G) AM1 and (H) AM3 in WT, oscerk1, OsCERK2ox;oscerk1 #6, #9, and #12 at 2 WAI. Error bars indicate the SD from 3 replicates for WT and oscerk1 with more than 5 replicates for both OsCERK2ox;oscerk1 and OsCERK2-DVox/oscerk1.