Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 6;607(7919):617–622. doi: 10.1038/s41586-022-04950-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 8 — a. Transient expression of Snv10H in N. benthamiana with co-infiltration of strychnine 10. Extracted ion chromatograms for 10-OH strychnine (m/z [M+H]⁺ = 351.1703 ± 0.05). This experiment was repeated three times with similar results. b. MS/MS (20 to 50 eV) spectra of 10-OH strychnine 12 produced in N. benthamiana (blue) compared to standard (red). c. Transient expression of Snv10H and SnvOMT in N. benthamiana with co-infiltration of strychnine 10. Extracted ion chromatograms for 10-OH strychnine (m/z [M+H]⁺ = 351.1703 ± 0.05, left) and β-colubrine 13 (m/z [M+H]⁺ = 365.1859 ± 0.05, right). This experiment was repeated three times with similar results. d. In vitro assays using purified SnvOMT from SoluBL21 E. coli with 10-OH strychnine 12. Extracted ion chromatograms for 10-OH strychnine (m/z [M+H]⁺ = 351.1703 ± 0.05, left) and β-colubrine 13 (m/z [M+H]⁺ = 365.1859 ± 0.05, right). This experiment was repeated more than three times with similar results. e. MS/MS (20 to 50 eV) spectra of β-colubrine 13 produced in N. benthamiana (blue) compared to standard (red). f. Reaction catalyzed by Snv10H and SnvOMT.